Fig. 1

Amifostine attenuates bleomycin-induced IPF. a Experimental design. C57BL mice were intratracheally injected with bleomycin on day 1. Three doses of intraperitoneal amifostine were administered on day 1, 3 and 5. Animals were sacrificed on day 7 or day 21, BAL and lung tissue samples were collected. b Animal body weights were measured every second day until day 14, n = 10, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. c Animal survival experiment was performed with high dose of bleomycin (5 U/kg). The survival rate was examined over 14 days n = 20, p < 0.001. d Histological H&E staining of lung tissues from control mice and from bleomycin-treated mice at days 7 and 21. Lung injury was evaluated as described in the Materials and Methods. n = 10, ****p < 0.0001. e The lung tissue fibrosis markers fibronectin, α-SMA and collagen1A1 were detected by Western blot. The results shown are cropped from original blots