Fig. 6

Effect of STAT3 overexpression and TASL silencing on LPS-induced inflammation and apoptosis in HK2 cells. A Western blot assay to detect the protein levels of STAT3, TASL, BCL-2, cleaved caspase-3, and BAX in LPS-induced HK2 cells in response to STAT3 overexpression and TASL silencing; B ELISA and qRT-PCR assay to detect the levels of IL6 and TNF-α in LPS-induced HK2 cells in response to STAT3 overexpression and TASL silencing. C Flow cytometry to detect apoptosis in LPS-induced HK2 cells in response to STAT3 overexpression and TASL silencing; D TUNEL staining to detect apoptosis in LPS-induced HK2 cells in response to STAT3 overexpression and TASL silencing (*P < 0.05, **P < 0.01, ***P < 0.001)