Fig. 5

Effect of silencing of STAT3 on LPS-induced inflammation and apoptosis in HK2 cells. A Western blot to detect the protein levels of STAT3, TASL, cleaved caspase-3, BCL-2, and BAX in LPS-induced HK2 cells after silencing of STAT3; B qRT-PCR to detect the mRNA expression of STAT3, TASL, BCL-2, and BAX in LPS-induced HK2 cells after silencing of STAT3; C ELISA to detect the levels of inflammatory factors IL6 and TNF-α in the supernatants of LPS-induced HK2 cells after silencing of STAT3; D qRT-PCR to detect the mRNA expression of IL6 and TNF-α in LPS-induced HK2 cells after silencing of STAT3; E TUNEL staining to detect the apoptosis in LPS-induced HK2 cells after silencing of STAT3 (*P < 0.05, **P < 0.01, ***P < 0.001)