Fig. 4

Effect of silencing of TASL on LPS-induced inflammation and apoptosis in HK2 cells. A, B, Protein (A) and mRNA (B) expression of TASL in HK2 cells after transfection with siTASL; C western blot to detect the protein levels of STAT3, TASL, BCL-2, cleaved caspase-3, and BAX in LPS-induced HK2 cells after silencing of TASL; D qRT-PCR to detect the mRNA expression of STAT3, TASL, BCL-2, and BAX in LPS-induced HK2 cells after silencing of TASL; E ELISA to detect the levels of inflammatory factors IL6 and TNF-α in the supernatants of LPS-induced HK2 cells after silencing of TASL; F qRT-PCR to detect the mRNA expression of inflammatory factors IL6 and TNF-α in the supernatants of LPS-induced HK2 cells after silencing of TASL; G TUNEL staining to detect apoptosis of in LPS-induced HK2 cells after silencing of TASL (*P < 0.05, **P < 0.01, ***P < 0.001)