Fig. 3

Effect of LPS induction on inflammation and apoptosis of HK2 cells. A Western blot to detect the protein levels of STAT3, TASL, BCL-2, cleaved caspase-3, and BAX in LPS-induced HK2 cells; B qRT-PCR to detect the mRNA expression of STAT3, TASL, BCL-2 and BAX in LPS-induced HK2 cells; C ELISA to detect the inflammatory factors IL6 and TNF-α levels in the supernatant of LPS-induced HK2 cells; D qRT-PCR to detect mRNA expression of IL6 and TNF-α in LPS-induced HK2 cells; E TUNEL staining to detect apoptosis in LPS-induced HK2 cells (**P < 0.01, ***P < 0.001)