Fig. 2

Bioinformatic prediction and experimental confirmation of the regulatory relationship between STAT3 and TASL. A STAT3 binding motif map in the TASL promoter region predicted by JASPAR; B detection of luciferase activity in HEK293T and HK2 cells after co-transfection of siSTAT3 and pSTAT3 with luciferase recombinant plasmid containing TASL gene promoter, respectively; C construction of the point mutant plasmid of two STAT3 binding sites in the TASL promoter region and determination of luciferase activity after transfection; D ChIP-qPCR and agarose gel electrophoresis assay for STAT3 binding in the TASL promoter region; E, F qRT-PCR for mRNA expression of STAT3 and TASL after transfection with siSTAT3 (E) and pSTAT3 (F); G, H western blot assay to detect the protein levels of STAT3 and TASL after transfection with siSTAT3 G and pSTAT3 H (**P < 0.01, ***P < 0.001, ****P < 0.0001)