Fig. 3
From: Neuroprotective effects of donepezil against Aβ25-35-induced neurotoxicity
Effects of donepezil (Donep) on the subcellular distribution of the PKCα and PKCε isoforms. PC 12 cells were treated with vehicle alone or with 10 μm donepezil for 2 h. Cytosolic and membrane proteins were then extracted separately for Western blot analysis. Donepezil decreased the expression of PKCα (from 100 to 74.83 ± 9.41, P < 0.01) and PKCε (from 100 to 79.75 ± 8.16, P < 0.05) in the cytosolic fraction but increased their expression (PKCα from 100 to 130.89 ± 12.45, P < 0.01; PKCε: from 100 to 148.39 ± 15.58, P < 0.001) in the membrane fraction