Fig. 2

Effect of hyaluronidase on three different HA-fillers in vitro. Time-lapse videomicroscopy fluorescence analysis of the HA-fillers a Juvederm Ultra 3 (JUV), b Belotero Balance Lidocain (BEL) and c Emervel classic (EMV) incubated with a fluorescent dye and bovine hyaluronidase (HYAL; blue dots) or control (NaCl; black circles) in hourly intervals over 20 h. d–u Representative fluorescence images obtained at 0 h (d–i), 10 h (j–o), and 20 h (p–u) for JUV (d, j, p with NaCl; e, k, q with HYAL), BEL (f, l, r with NaCl; g, m, s with HYAL), and EMV (h, n, t with NaCl; j, o, u with HYAL). Red asterisks mark time points showing statistically significant differences of fluorescence intensity between two groups (*p < 0.05, **p < 0.01). v Summary of fluorescence intensities for JUV (blue dots), BEL (red dots), EMV (green dots) normalized against their respective NaCL-CTRs (zero line)