From: Influence of proinflammatory cytokine gene polymorphism on childhood obesity
PCR stage | IL-6 | TNF | IL-1 |
---|---|---|---|
Initial denaturation | 94°C 10 min | 94°C 3 min | 94°C 4 min |
Denaturation | 94°C 1 min | 94°C 1 min | 94°C 30 s |
Annealing | 55°C 35 s | 60°C 1 min | 63°C 30 s |
Elongation | 72°C 1 min | 72°C 1 min | 72°C 30 s |
Cycles number | 35 | 36 | 35 |
Final elongation | 72°C 10 min | 72°C 5 min | 72°C 10 min |