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Fig. 4 | European Journal of Medical Research

Fig. 4

From: Thrombin-activated platelet-rich plasma enhances osteogenic differentiation of human periodontal ligament stem cells by activating SIRT1-mediated autophagy

Fig. 4

Effects of autophagy inhibition on activated PRP-induced hPDLSC proliferation and ALP activity. hPDLSCs were treated with 20% thrombin-activated PRP or together with sirtinol or 3-MA. A MTT assay results of hPDLSC proliferation. B ALP staining of PRP-treated hPDLSCs. C Western blotting analyses and quantitative analysis demonstrated the expression of SIRT1, LC3-I, LC3-II and Beclin-1. GAPDH was used as an internal control. *P < 0.05, **P < 0.01, ***P < 0.001

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